【摘 要】
：
该研究旨在探讨苯并(a)芘[Benzo(a)pyrene,B(a)P]对孕早期小鼠卵巢黄体功能的影响及机制.体内模型:将昆明小鼠每晚按雌雄3∶1的比例合笼,次晨查得阴栓记为孕第1天(d1);将其随
【机 构】
：
重庆医科大学公共卫生与管理学院生殖生物学研究室,重庆医科大学生殖与发育国际合作联合实验室,重庆400016
论文部分内容阅读
该研究旨在探讨苯并(a)芘[Benzo(a)pyrene,B(a)P]对孕早期小鼠卵巢黄体功能的影响及机制.体内模型:将昆明小鼠每晚按雌雄3∶1的比例合笼,次晨查得阴栓记为孕第1天(d1);将其随机分为对照组和B(a)P处理组,每日早晨称重后以0.1 mL/10 g动物体质量灌胃给予0.2 mg/(kg·d)的B(a)P,对照组灌胃等体积的玉米油,收取d4、d7小鼠卵巢组织.体外模型:培养小鼠卵巢颗粒细胞KK-1,将其分为对照组(0.1％DMSO)、HCG组(1.0 IU/mL HCG)、HCG+BPDE(1.0 IU/mL HCG和0.5 μmol/L BPDE)联合处理组,处理细胞24 h后进行后续检测.ELISA检测小鼠血清雌激素(E2)、孕激素(P4)水平;qRT-PCR检测体内外卵巢雌、孕激素合成限速酶3β-HSD、17β-HSD和P450SCC的mRNA水平;免疫组化检测卵巢组织切片中Ki67、PCNA的表达,CCK-8检测KK-1细胞增殖情况;Western blot、免疫组化和免疫荧光检测周期相关蛋白CyclinA1、CDK2、CDK4、CyclinB1以及GAS1的表达情况.透射电镜和Mitotracker探针观察线粒体形态.与对照组相比,B(a)P暴露导致孕早期小鼠血清中E2、P4水平明显降低;同时,卵巢雌、孕激素合成限速酶3β-HSD、17β-HSD和P450SCC mRNA水平下调;CCK-8结果显示,BPDE暴露导致细胞活力下降;体内B(a)P暴露导致卵巢黄体中Ki67、PCNA表达下调;Western blot、免疫组化和免疫荧光结果显示,B(a)P或BPDE暴露下调细胞周期相关因子CyclinA1、CDK2、CDK4、CyclinB1及GAS1水平;电镜和免疫荧光结果显示,BPDE暴露导致线粒体形态异常.B(a)P及其代谢物BPDE干扰细胞周期调控,影响线粒体功能,进而导致孕早期小鼠卵巢黄体功能异常.
其他文献
Aging is a major risk factor for many diseases,especially in highly prevalent cardiopulmonary comorbidities and infectious diseases including Coronavirus Diseas
Calcium-dependent proteolytic calpains are implicated in a variety of physiological processes,as well as pathologies associated with calcium overload.However,th
The human calcium-sensing receptor(CaSR)is a class C G protein-coupled receptor(GPCR)responsible for maintaining Ca2+homeostasis in the blood.The general consen
The impact of the microenvironment on innate lymphoid cell (ILC)-mediated immunity in humans remains largely unknown.Here we used full-length Smart-seq2 single-
Interactions with RNA-binding proteins (RBPs) are integral to RNA function and cellular regulation,and dynamically reflect specific cellular conditions.However,
Nerve-cancer interactions modulate pancreatic cancer growth through direct effects on cancer cells and the microenviron-ment.New evidence now shows that nerves
该文研究了N-乙酰半乳糖氨基转移酶6(GALNT6)对乳腺癌上皮-间充质转化(EMT)的影响及其机制.乳腺癌细胞中GALNT6表达被敲低后,间充质标志物E-cadherin表达增加,N-cadherin、Vi
RNase Ⅲ DROSHA is upregulated in multiple cancers and contributes to tumor progression by hitherto unclear mechanisms.Here,we demonstrate that DROSHA interacts
该文探讨了缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)在新生儿坏死性小肠结肠炎(neonatal necrotizing enterocolitis,NEC)中的作用机制.收集8例NEC患儿小肠
Dear Editor,rnThe ongoing coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has resulted in an