论文部分内容阅读
目的:观察白细胞介素(IL)-4对重型肝炎患者外周血单个核细胞(PBMCs)肿瘤死因子-α(TNF-α)表达的影响,以了解 IL-4在重型肝炎治疗中的潜在应用价值。方法:应用 Logo 细胞毒试验、免疫组织化学法和半定量逆转录-多聚酶链反应(RT-PCR)检测 TNF-α表达水平。结果:IL-4在蛋白质和 mRNA 水平明显抑制脂多糖(LPS)诱导的亚急性和慢性重型肝炎患者 PBMCs TNF-α的产生,这种抑制作用呈剂量和时间依赖的方式。IL-4于100U/ml 时开始出现抑制效应,于1000U/ml 时抑制作用接近最大。虽然 IL-4对慢性重型肝炎 PBMCs TNF-α的抑制作用略低,但与未经 IL-4处理的 PBMCs 相比较,TNF-α的表达水平仍有显著下降。结论:IL-4是一种有效的 TNF-α下向调节因子,在重型肝炎的治疗上具有潜在的应用价值。
Objective: To investigate the effect of interleukin-4 on the expression of tumor necrosis factor-α (TNF-α) in peripheral blood mononuclear cells (PBMCs) of patients with severe hepatitis to find out the potential application of IL-4 in the treatment of severe hepatitis value. Methods: Logo cytotoxicity assay, immunohistochemistry and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the expression of TNF-α. Results: IL-4 at mRNA and protein levels significantly inhibited the production of TNF-α in PBMCs induced by lipopolysaccharide (LPS) in subacute and chronic severe hepatitis patients in a dose-and time-dependent manner. IL-4 began to appear in 100U / ml inhibitory effect, inhibition at 1000U / ml close to maximum. Although the inhibitory effect of IL-4 on TNF-α in PBMCs of chronic severe hepatitis was slightly lower, the expression level of TNF-α was still significantly lower than that of PBMCs without IL-4 treatment. Conclusion: IL-4 is a potent down-regulation factor of TNF-α and has potential value in the treatment of severe hepatitis.