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目的观察重复 +Gz暴露是否引起心肌组织儿茶酚胺类物质代谢异常以及低G预适应和天然抗氧化剂茶多酚 (TP)的防护作用。方法 32只雄性Wistar大鼠随机分为 4组 (n =8) :A组 (对照组 ) ,仅受到 + 1Gz作用 5min ;B组 ( +Gz应激组 ) ,+ 1 0Gz峰值暴露 30s 次 ,重复 5次 d ,间隔为 + 1Gz 1min ,3d wk ,共 3wk ;C组 (低G预适应组 ) ,+Gz应激条件同B组 ,但 + 1 0Gz暴露前 1h受到 + 2Gz作用 5min ;D组 (TP防护组 ) ,+Gz应激条件同B组 ,但 + 1 0Gz暴露前 1h灌胃给予茶多酚 2 0 0mg kg。于末次 +Gz暴露的次日上午将各组大鼠断头处死 ,迅速摘取心脏置液氮冷藏 ,用高效液相色谱电化学检测法测定各组大鼠心肌儿茶酚胺类物质含量 ,包括去甲肾上腺素 (NA) ,肾上腺素 (AD) ,多巴胺(DA) ,5 羟色胺 ( 5 HT)。结果与A组相比 ,B组心肌AD水平显著升高 (P <0 .0 1 ) ,但NA ,DA ,5 HT含量无明显差别。与B组相比 ,C、D组心肌AD、5 HT含量明显降低 ,而D组DA水平显著升高。结论重复 + 1 0Gz暴露引起大鼠心肌儿茶酚胺类物质代谢异常 ,主要是AD水平显著升高 ;低G预适应和天然抗氧化剂TP具有明显的防护作用
Objective To observe whether repeated + Gz exposure caused myocardial metabolism of catecholamines and the protective effect of low G preconditioning and natural antioxidant tea polyphenols (TP). Methods 32 male Wistar rats were randomly divided into 4 groups (n = 8): group A (control group), which received + 1Gz only for 5 minutes; group B (+ Gz stress group) Group C (low G preconditioning group), + Gz stress group were as same as group B, but +10 Gz was exposed to +2 gz for 5 min 1h before exposure; D Group (TP protection group), + Gz stress conditions with the B group, but + 1 0Gz 1h before exposure to give tea polyphenols 200mg kg. The rats in each group were decapitated on the morning after the last + Gz exposure, and the hearts were promptly removed and stored in cold nitrogen. The contents of catecholamines in myocardium of rats in each group were determined by high performance liquid chromatography (electrochemical detection) Epinephrine (NA), epinephrine (AD), dopamine (DA), serotonin (5 HT). Results Compared with group A, the level of myocardial AD in group B was significantly increased (P <0.01), but the content of NA, DA and 5 HT had no significant difference. Compared with group B, the levels of myocardial AD and 5 HT in group C and group D were significantly decreased, while the level of DA in group D was significantly increased. Conclusions Repeated +10Gz exposure can induce myocardial catecholamine metabolism in rats, mainly due to a significant increase in AD levels; low G preconditioning and natural antioxidant TP have significant protective effects