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目的:建立路路通胶囊的质量控制方法。方法:用薄层色谱法对三七、赤芍、当归、丹参进行定性鉴别;高效液相色谱法测定路路通胶囊中阿魏酸的含量,采用Diamonsil C_(18)柱(250 mm×4.6 mm,5μm),以甲醇-0.1%磷酸溶液(30:70)为流动相;流速为1.0 ml·min~(-1);柱温:25℃;检测波长为320 nm。结果:TLC法检出了三七、赤芍、当归、丹参特征斑点,阿魏酸进样量在0.010～0.103μg范围内线性关系良好,r=1.000 0,平均加样回收率为99.77%,RSD为0.93%(n=9)。结论:所建立的方法简便、稳定、准确、重复性好,可作为本品的质量控制方法。
Objective: To establish a quality control method of Lilutong capsule. Methods: The contents of ferulic acid in Raluxotong capsules were determined by TLC. The Diamonsil C 18 column (250 mm × 4.6) was used for the qualitative identification of Panax notoginseng, Radix Paeoniae Rubra, Radix Angelicae Sinensis and Radix Salviae Miltiorrhizae. The mobile phase was methanol-0.1% phosphoric acid solution (30:70) at a flow rate of 1.0 ml · min -1. The column temperature was 25 ℃ and the detection wavelength was 320 nm. Results: The spots of Panax notoginseng, Radix Paeoniae Rubra, Angelicae Sinensis and Salvia miltiorrhiza were detected by TLC method. The calibration curve of ferulic acid was linear in the range of 0.010-0.103 μg with r = 1.000 0 and the average recoveries were 99.77% RSD was 0.93% (n = 9). Conclusion: The established method is simple, stable, accurate, reproducible, and can be used as the quality control method of this product.