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目的探讨两起同地区发生的金黄色葡萄球菌食物中毒分离株之间的分子流行病学关系。方法参照国家标准进行金黄色葡萄球菌分离培养与鉴定,用PCR方法和脉冲场凝胶电泳技术(PFGE)分析分离菌株的分子流行病学特征,采用微量肉汤稀释法进行药物敏感试验。结果共检出15株金黄色葡萄球菌,其中病人生物标本检出6株,剩余食品中9株,烤鸡肉中金黄色葡萄球菌含量最高;葡萄球菌肠毒素均为SEA型,同一事件的菌株为同一克隆株;两起事件的菌株PFGE型别不一致,药敏结果也有差异。结论两起SEA型葡萄球菌肠毒素引起的食物中毒,由不同来源的金黄色葡萄球菌污染所导致。
Objective To investigate the molecular epidemiological relationship between two Staphylococcus aureus food poisoning isolates in two regions. Methods The isolation and culture of Staphylococcus aureus were carried out with reference to national standards. The molecular epidemiological characteristics of the isolated strains were analyzed by PCR and PFGE, and the drug sensitivity test was carried out by using the broth microdilution method. Results A total of 15 strains of Staphylococcus aureus were detected, of which 6 were detected in the patient’s biological samples and 9 in the remaining food samples. The content of Staphylococcus aureus was the highest in roasted chicken. Staphylococcal enterotoxins were all SEA-type. The same strain was The same clonal strain; PFGE types of the two incidents are inconsistent, the drug sensitivity results are also different. Conclusions Two food poisonings caused by two SEA-type staphylococcal enterotoxins result from the contamination of Staphylococcus aureus from different sources.