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目的： 了解ｒｈ Ｔ Ｎ Ｆα Ｄｂ５０ Ｇ 体内体外抗肿瘤活性。方法： 体外采用 Ｍ Ｔ Ｔ 法测ｒｈ Ｔ Ｎ Ｆα Ｄｂ５０ Ｇ 对 Ｍ Ｋ Ｎ４５ 细胞的 Ｉ Ｃ５０ ， 体内采用动物移植性肿瘤检测其抗肿瘤活性。结果： Ｍ Ｔ Ｔ法测定结果表明ｒｈ Ｔ Ｎ Ｆα Ｄｂ５０ Ｇ 对一定浓度的人胃癌细胞株 Ｍ Ｋ Ｎ４５ 有较强的抑制增殖作用， 其 Ｉ Ｃ５０ 为４ ．４６１ ×１０ － ２μｇ／ ｍｌ（ 相关系数γ＝ ０ ．９５４６） 。体内研究表明ｒｈ Ｔ Ｎ Ｆα Ｄｂ５０ Ｇ 对小鼠 Ｓ１８０ 实体瘤和小鼠 Ｅｈｒｌｉｃｈ 氏实体瘤有较强的抑制作用，其抑制率分别为２９ ．３ ％ （ｉｖ） 和４０ ．０７ ％ （ｉｖ） 。ｒｈ Ｔ Ｎ Ｆα Ｄｂ５０ Ｇ 对小鼠黑色素瘤 Ｂ１６ 的肺转移有很强的抑制作用， 其抑制率为６０ ．４１ ％ （ｉｖ） 。结论： 人重组肿瘤坏死因子ｒｈ Ｔ Ｎ Ｆα Ｄｂ５０ Ｇ 在体内体外均有一定的抗肿瘤作用。
Objective: To understand the anti-tumor activity of rh TN FαDb50 G in vitro and in vivo. METHODS: In vitro, MTT method was used to measure the I C50 of rhNNAFαDb50G against MKN45 cells. Animal transplanted tumors were used to examine their antitumor activity. RESULTS: The MTT assay results showed that rh-TN Fα-Db-50G had a strong inhibitory effect on the proliferation of MKN-45 human gastric cancer cell lines, and its I C50 was 4. 461 × 10 - 2μg/ml (correlation coefficient γ = 0 .9546). In vivo studies showed that rh-TN FαDb50 G had a strong inhibitory effect on S180 solid tumors in mice and Ehrlich solid tumors in mice. The inhibition rates were 29. 3% (iv) and 40. 07 % (iv). Rh T N Fα Db 50 G has a strong inhibitory effect on the lung metastasis of mouse melanoma B16 with an inhibition rate of 60. 41% (iv). Conclusion: Human recombinant tumor necrosis factor rh T N Fα Db 50 G has a certain anti-tumor effect in vitro and in vivo.