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抗丁氏双鳍电鳐电器官乙酰胆碱酯酶（ＡＣｈＥ）的抑制性单克隆抗体ＩＨ１１（ＩｇＭ），丁氏双鳍电鳐，石纹电线电器官，比目鱼肌肉，大鼠脑，牛脑，人脑，牛红细胞及人红细胞ＡＣｂＥ均以不同的亲和层析法提纯。用酶抗原免疫测定法及酶联免疫吸附测定法检测了１Ｈ１１与上述脊椎动物的ＡＣｈＥ的交叉免疫反应性．结果表明，１Ｈ１１可与石纹电绥，比目鱼肌肉，大鼠脑、牛脑及人脑ＡＣｈＥ有交叉免疫反应。显示了１Ｈ１１所针对的抗原决定簇在进化中的高度保守性。１Ｈ１１与人，牛红细胞ＡＣｈＥ及人丁酰胆碱酯酶（ＢＣｈＥ）无交叉免疫反应性。提示哺乳类脑ＡＣｈＥ与红细胞ＡＣｈＥ及人ＢＣｈＥ有不同的抗原决定簇。１Ｈ１１对电鳐及电鳗ＡＣｈＥ活性产生明显抑制。但对其它几个脊椎动物的ＡＣｈＥ活性无抑制．表明１Ｈ１１所针对的抗原决定簇在电鳐ＡＣｈＥ的三级结构中靠近其活性中心，而在其它脊椎动物的ＡＣｈＥ中刚远离其活性中心．ＡＣｈＥ阴离子部位抑制剂依酚氟铵不影响抗原抗体反应，表明１Ｈ１１不结合在阴离子部位。Ｎ－糖苷酶Ｆ处理使ＡＣｂＥ去糖基化后。１Ｈ１１不再能与ＡＣｈＥ结合，表明至少１Ｈ１１所识别的抗原决定簇的主要部分是Ｎ－多醣．抑制性单克隆抗体１Ｈ１１对脑ＡＣｈ?
Anti-Dichotoma bifidus Electrical Potential Inhibitors of AChE Monoclonal Antibodies IH11 (IgM), Dichotomous Dichotomous, Staurotline Electrical, Soleus Muscle, Rat Brain, Bovine Brain, Human Brain, bovine red blood cells and human erythrocyte ACbE were purified by different affinity chromatography. The cross-reactivity of 1H11 with AChE of vertebrates was tested by enzyme immunoassay and enzyme-linked immunosorbent assay (ELISA). The results showed that 1H11 could cross-react with AChE, Ishikari, soleus muscle, rat brain, bovine brain and human brain. The antigenic determinants against 1H11 are highly conserved in evolution. 1H11 has no cross-reactivity with human, bovine red blood cell AChE and human butyrylcholinesterase (BChE). Tip mammalian brain AChE and red blood cells AChE and human BChE have different antigenic determinants. 1H11 significantly inhibited the activity of AChE in electric and electric eels. But did not inhibit AChE activity of several other vertebrates. Indicating that the epitope targeted by 1H11 is close to its active site in the tertiary structure of AChE, and far away from its active site in AChE of other vertebrates. AChE anion site inhibitor phenphenamine did not affect the antigen-antibody reaction, indicating that 1H11 does not bind to the anion site. After N-glycosidase F treatment deglycosylated ACbE. 1H11 no longer binds to AChE, indicating that at least the major portion of the epitope recognized by 1H11 is N-polysaccharide. Inhibitory monoclonal antibody 1H11 on brain ACh?