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目的:探讨子痫前期(PE)患者胎盘组织血管内皮生长因子(VEGF)和抑癌基因p53的表达变化、相互关系及其在PE发病过程中的作用。方法:采用链酶菌抗生物素蛋白-过氧化物酶连接(SP)法检测35例PE患者(轻度15例和重度20例)和25例正常妊娠(对照组)胎盘组织中VEGF和p53的表达变化。结果:(1)重度PE患者胎盘组织中VEGF表达强度显著低于正常对照组和轻度PE组(P均<0.05),而轻度PE组与正常对照组无显著差异(P>0.05);(2)PE患者胎盘组织中p53的表达强度较正常对照组均显著升高(P<0.05),重度PE组亦显著高于轻度PE组(P<0.05);(3)胎盘组织中VEGF和p53的表达呈显著负相关(r=-0.428,P=0.001)。结论:VEGF和p53在PE和正常妊娠胎盘组织中的表达变化预示着其与PE的发生、发展有一定的关系。
Objective: To investigate the expression of vascular endothelial growth factor (VEGF) and tumor suppressor gene p53 in placental tissue of patients with preeclampsia (PE) and their relationship and their roles in the pathogenesis of PE. Methods: The levels of VEGF and p53 in placenta of 35 PE patients (15 mild cases and 20 severe cases) and 25 normal pregnant controls (control group) were detected by streptavidin-peroxidase (SP) Changes in expression. Results: (1) The expression of VEGF in severe PE patients was significantly lower than that in normal controls and mild PE patients (P <0.05), while there was no significant difference between mild PE patients and normal controls (P> 0.05). (2) The expression intensity of p53 in placental tissue of PE patients was significantly higher than that of the normal control group (P <0.05), and also significantly higher in the severe PE group than in the mild PE group (P <0.05). (3) And p53 expression was significantly negatively correlated (r = -0.428, P = 0.001). Conclusion: The expression changes of VEGF and p53 in placenta of PE and normal pregnancy indicate that PE and VEGF have a certain relationship with the occurrence and development of PE.