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针对剧变态的中国林蛙幼蛙雌雄不易鉴定的问题，本研究找出了幼蛙的固定、生殖腺的剖取、性腺组织切片的制作过程及性别鉴定方法。
In view of the problem that the metamorphosis of Chinese frog frogs are not easily identified by both sexes, this study identified the immobilization of the frogs, the dissection of the gonads, the process of making the gonadal tissue slices, and the sex determination method.