将光谱精确定量分析特性与图像定位检测特性相结合,形成新颖的综合分析技术,是光谱成像技术的目标和特征。本文介绍光谱成像的原理和我们研制的显微荧光光谱成像系统,及其在生物医学领域的若干应用实例。实验证明,光谱成像技术是有广泛应用前景的综合分析技术。 It is the target and characteristic of spectral imaging technology to combine spectral precise quantitative analysis with image localization detection to form a novel integrated analysis technique. This article introduces the principle of spectral imaging and the microscopic fluorescence spectroscopy imaging system we have developed and its application examples in the field of biomedicine. Experiments show that spectral imaging technology is a comprehensive analysis technology with broad application prospects.