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紫膜(PM)表面的极性磷脂和带电氨基酸所构成的不对称电荷结构对紫膜的结构功能有重要意义.Jonas等指出在pH5～9时,如果不考虑阳离子结合在紫膜表面的羧基上,则单位菌紫质(BR)的紫膜表面总负电荷数的理论值为19,当阳离子结合后将显著降低.遗憾的是大部分实验值在pH7时在2～10之间波动.虽然有利于阳离子结合模型,但由于大部分实验测量方法依赖于Gouy-Chapman理论,因而所测值不可避免地受所取离于强度不同和膜
The asymmetric charge structure of polar phospholipids and charged amino acids on the surface of the purple membrane (PM) is of great importance to the structural function of the purple membrane. Jonas et al. Showed that at pH 5-9, if the carboxyl groups on the surface of the purple membrane , The theoretical value of the total negative charge on the purple membrane surface of the unit bacteriorhodopsin (BR) is 19, which decreases significantly when the cation is bound.Most of the experimental values fluctuate between 2 and 10 at pH7. Although favorable for the cation binding model, since most experimental measurements rely on the Gouy-Chapman theory, the measured values inevitably suffer from dissociation from strength and membrane