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目的：观察中等强度持续训练后骨骼肌线粒体抗氧化能力和ATP酶活性的变化。方法 SD大鼠24只，8只为正常对照组（A组），16只采用递增负荷训练建立中等强度持续运动跑台训练模型，又均分为运动2周及6周组（B及C组）。B及C组大鼠按Bradford等的方法分别训练2和6周，每周训练6 d。训练结束后大鼠处死，用差速离心法提取腓肠肌线粒体，紫外-可见分光光度计检测锰超氧化物歧化酶（Mn-SOD）活性；脂质过氧化产物丙二醛(MDA)含量采用TBA（硫代巴比妥酸）法测定；Na+K+-ATP酶和Ca2+Mg2+-ATP酶活性采用酶促反应定磷法测定。结果B组的骨骼肌线粒体Mn-SOD水平与A组相比差异无统计学意义，P>0.05，而MDA含量显著高于对照组，P<0.05。C组的Mn-SOD水平显著高于A组， P<0.05，而MDA含量显著低于A组，P<0.05。B组Na+K+-ATP酶和Ca2+Mg2+-ATP酶活性均显著低于A组，均P<0.05，而C组的Na+K+-ATP酶和Ca2+Mg2+-ATP酶活性均显著高于A组，均P0.05, whereas the content of MDA of Group C was significantly higher than that of Group A ,P<0.05. The Mn-SOD level of Group C was significantly higher than that of Group A,P<0.05, however, the content of MDA of Group C was signiuficantly lower than that of Groupo A ,P<0.05. The activities of Na+K+-ATPase and Ca2+Mg2+-ATPase of Group B were both significantly lower than those of Group A,bothP<0.05, however, those of Group C were bothsignificantly higher than that of Group A ,P <0.05. Conclusion A certain period of moderate-intensity continuous training increases the activity of Mn-SOD, decreases the level of lipid peroxidation, reduces the injury of free radical, enhances the activity of ATPase, and improves the level of the energy metabolism in skeletal muscle mitochondria .