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目的探讨肝激酶B1(LKB1)在氢醌诱导下,在小鼠骨髓造血干细胞(BM-HSC)与胚胎造血干细胞(YS-HSC)中的表达情况。方法免疫磁珠法提取小鼠BM-HSC,与YS-HSC,分别接种于含不同浓度氢醌(0、1.25、2.5、5.0、10μmol/L)的培养基中,检测细胞活力、LKB1 mRNA及蛋白表达、细胞内活性氧(ROS)和细胞凋亡。结果 BM-HSC的1.25、2.5、5、10μmol/L氢醌组LKB1相对表达量分别为(1.27±0.057)、(1.18±0.021)、(0.89±0.009)、(0.68±0.006),YS-HSC的LKB1相对表达量分别为(1.33±0.024)、(0.96±0.017)、(0.7±0.008)、(0.54±0.022),组间比较,差异均有统计学意义(P<0.05);HQ诱导后LKB1蛋白水平与mRNA水平表达一致,凋亡率与LKB1表达结果一致。结论 HQ能够影响小鼠BM-HSC与YS-HSC的LKB1表达,HQ对于小鼠造血干细胞的氧化应激效应与LKB1活性相关。
Objective To investigate the expression of hepatic kinase B1 (LKB1) induced by hydroquinone in mouse bone marrow hematopoietic stem cells (BM-HSCs) and fetal hematopoietic stem cells (YS-HSC). Methods BM-HSC and YS-HSC were isolated by magnetic beads method. The cells were inoculated into medium containing different concentrations of hydroquinone (0, 1.25, 2.5, 5.0 and 10μmol / L) Protein expression, intracellular reactive oxygen species (ROS) and apoptosis. Results The relative expressions of LKB1 in 1.25, 2.5, 5, 10μmol / L hydroquinone group were (1.27 ± 0.057), (1.18 ± 0.021), (0.89 ± 0.009), (0.68 ± 0.006) (1.33 ± 0.024), (0.96 ± 0.017), (0.7 ± 0.008) and (0.54 ± 0.022), respectively. The differences between the two groups were statistically significant (P <0.05) The expression of LKB1 protein was consistent with the mRNA level, and the apoptosis rate was consistent with the expression of LKB1. Conclusion HQ can affect the expression of LKB1 in BM-HSC and YS-HSC. The oxidative stress induced by HQ is related to the activity of LKB1 in mouse hematopoietic stem cells.