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建立实验性自身免疫性肝炎 (EAH)小鼠模型 ,并对其进行动态观察。方法 以同种系S 10 0肝抗原与弗氏完全佐剂充分乳化后两次予小鼠腹腔注射 ,并设单纯磷酸盐缓冲液 (PBS)、S 10 0肝抗原和弗氏完全佐剂处理对照组。将S 10 0肝抗原层析分离后观察自身抗原特异性T细胞增殖反应。结果 模型组小鼠在首次注射后 2周即可见到多形核细胞特别是淋巴细胞的浸润。首次注射后 4周组织学改变达高峰 ,组织学病变的消退较慢 ;血清ALT水平的动态变化与组织学改变相似 ;模型组存在自身抗原特异性T细胞增殖反应。结论 实验性自身免疫性肝炎是一个可能由自身反应性T细胞介导的自身免疫性肝炎的小鼠模型 ,可用于研究自身免疫性肝炎的发病机制
To establish a mouse model of experimental autoimmune hepatitis (EAH), and to observe it dynamically. Methods The mice were injected intraperitoneally twice with the same kind of S 10 0 liver antigen and Freund’s complete adjuvant, and then treated with phosphate buffered saline (PBS), S 10 0 liver antigen and Freund’s complete adjuvant Control group. S 10 0 liver antigen was separated and observed for autoantigen-specific T cell proliferation. Results The mice in model group showed infiltration of polymorphonuclear cells, especially lymphocytes, two weeks after the first injection. At 4 weeks after the first injection, histological changes reached its peak, histological lesions subsided more slowly. The dynamic changes of serum ALT levels were similar to histological changes. There was autoantigen-specific T cell proliferative response in model group. Conclusions Experimental autoimmune hepatitis is a mouse model of autoimmune hepatitis that may be mediated by autoreactive T cells and may be used to study the pathogenesis of autoimmune hepatitis