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常规石蜡切片的标本采用甲醛固定,但蛋白质分子交联增多,封闭抗原,影响免疫组化检测结果。家用压力锅适用于常规福尔马林固定石蜡包埋组织的抗原修复或暴露,使得免疫组化结果更加可靠。1 材料和方法1.1 材料:用辽宁生产的双喜牌多保险防爆安全压力锅,1000w电炉,不锈钢托架。试剂由迈新公司提供的即用型LCA、KI-67、ER、PR及S-P试剂盒,收集1996年～1999年本院手术标本,经防脱水处理载玻片制成切片备用共三组。1.2 操作方法:(1)常规组织切片经常规二甲苯脱蜡,梯度酒精脱水,浸泡于PBS中待用。第一组使用不锈钢压力锅处理;第二组使用微波处理;第三组不经上述处理;(2)抗原修复:取1500～3000ml的PH=6.0柠檬酸盐缓冲液于压力锅中,放电炉加热直至沸腾,将脱蜡水化后的组织切片置于不锈钢托架上,放入已沸腾的缓冲液中,盖上锅盖,扣上压力阀,继续大火
Formal paraffin sections were fixed with formaldehyde, but the protein molecules increased cross-linking, blocking antigen, affecting the results of immunohistochemistry. Home pressure cooker for routine formalin fixed paraffin embedded tissue antigen retrieval or exposure, making immunohistochemistry results more reliable. 1 Materials and methods 1.1 Materials: Double-brand fire safety explosion-proof pressure cooker produced in Liaoning, 1000w electric furnace, stainless steel bracket. Reagents The ready-to-use LCA, KI-67, ER, PR and S-P kits provided by Mai Sun Company were used to collect surgical specimens of our hospital from 1996 to 1999 and prepared into slices for spare three generations after dehydration-treated slides. 1.2 Methods of Operation: (1) conventional tissue sections conventional xylene dewaxing, gradient alcohol dehydration, immersion in PBS stand-by. The first group is treated with stainless steel pressure cooker; the second group is treated with microwave; the third group is not treated as above; (2) antigen recovery: Take 1500 ~ 3000ml pH = 6.0 citrate buffer in a pressure cooker, Boiling, the dewaxing hydration of tissue slices placed on a stainless steel bracket, into the boiling buffer, cover the lid, buckle the pressure valve, continue the fire