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目的初步探讨固有淋巴细胞(ILCs)亚群如ILC1和ILC2(分别为适应性免疫应答中Th1和Th2的映像)对病原体感染的作用机制。方法收集临床慢性乙肝(CHB)患者和健康对照外周血和肝组织,运用流式细胞术、定量PCR和ELISA方法,检测CHB患者中ILC1和ILC2的频率、Ⅰ型和Ⅱ型转录因子和细胞因子水平,并与CHB患者临床指标进行相关性分析。结果CHB患者总ILCs中,ILC1转录因子T-bet、效应细胞因子IFN-γ、ILC1分化相关信号通路分子IL-12/IL-12R水平均显著升高。升高的ILC1亚群频率与CHB患者肝损伤显著相关,但与替比夫定疗效无明显关联。虽然ILC2相关的转录因子、细胞因子等在CHB中也升高,但升高的幅度均低于ILC1细胞。而且,ILC2细胞活性与HBV拷贝或肝损伤均没有显著关联。结论本研究结果提示了ILC1在CHB病理过程中的潜在促炎作用,以及ILC1及其相关分子可能是CHB诊断、预后甚至治疗的潜在干预靶标。
OBJECTIVE: To investigate the mechanism of action of innate lymphocyte (ILCs) subsets such as ILC1 and ILC2 (maps of Th1 and Th2 in adaptive immune responses, respectively) on pathogen infection. Methods Chronic hepatitis B (CHB) patients and healthy controls were collected from peripheral blood and liver tissues. The frequencies of ILC1 and ILC2 in CHB patients were detected by flow cytometry, quantitative PCR and ELISA. The expressions of type Ⅰ and Ⅱ transcription factors and cytokines Level, and with CHB patients with clinical indicators related analysis. Results The levels of ILC1 transcription factor T-bet, IFN-γ, IL-12 / IL-12R in ILC1 were significantly increased in all the patients with CHB. Elevated ILC1 subpopulation frequency was significantly associated with liver injury in patients with CHB but not with telbivudine efficacy. Although ILC2-associated transcription factors, cytokines and others were also elevated in CHB, the magnitude of increase was lower than that of ILC1 cells. Moreover, ILC2 cell activity was not significantly associated with HBV copies or liver damage. Conclusions The results of this study suggest a potential proinflammatory role of ILC1 in CHB pathology and that ILC1 and its related molecules may be potential targets for diagnosis, prognosis and even treatment of CHB.