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在 16m3气雾室中 ,采用Devibiss气溶胶发生器 ,在 0 0 3~ 0 0 4MPa压力下 ,以 0 8m3/h流量喷含 1 0 %蛋白胨的白色葡萄球菌菌悬液形成气溶胶。结果 ,直径 <4 7μm的气溶胶粒子平均占 82 57%。在室温 2 0~ 2 5℃ ,相对湿度为 55%~ 70 %条件下 ,4 0min内其自然衰亡率不超过 38 0 0 % ,能维持试验菌数为 5× 10 4 ~ 5× 10 5cfu/m3。自然菌试验表明 ,采空气自然菌的平板 ,培养 72h比 4 8h为好。
In a 16m3 aerosol chamber, a 10% peptone suspension of Staphylococcus aureus was sprayed at a flow rate of 0 8 m3 / h using a Devibiss aerosol generator at a pressure of 0 0 to 0 0 4 MPa to form an aerosol. As a result, aerosol particles with diameters <47 μm averaged 82 57%. Under the conditions of room temperature 20 ~ 25 ℃ and relative humidity 55% ~ 70%, the natural decay rate did not exceed 38 0 0% within 40 min, and the number of tested bacteria could be maintained at 5 × 10 4 ~ 5 × 10 5 cfu / m3. Natural bacteria test showed that the air natural bacteria plate, culture 72h better than 48h.