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介绍了一种基于芯片的微型聚合酶链式反应(polymerase chain reaction,PCR)系统,利用该系统进行了温度控制和响应特性研究。系统由PCR芯片、封装PCB板及单片机控制系统组成。PCR芯片采用MEMS技术制作。用微型PCR系统对芯片上含有微加热器的反应仓进行了升温降温、循环温度和液体负载对比加热等实验。结果显示:微加热器升温速度可以达到3℃/s,降温速度可以达到1℃/s,稳定后温度变化小于0.1℃。通过实验结果与理论计算的对比,分析了微加热器的最高温度与加热功率之间的对应关系及提高温控特性的关键因素。
A chip-based micro-polymerase chain reaction (PCR) system is introduced, and the temperature control and response characteristics of the system are studied. System consists of PCR chip, package PCB board and SCM control system. PCR chip using MEMS technology. Micro-PCR system on the chip containing micro-heater reaction chamber was heated and cooled, circulating temperature and liquid load contrast heating experiments. The results show that the heating rate of micro-heater can reach 3 ℃ / s, the cooling rate can reach 1 ℃ / s, and the temperature change is less than 0.1 ℃ after stabilization. By comparing the experimental results with theoretical calculations, the corresponding relationship between the maximum temperature and the heating power of micro-heaters and the key factors to improve the temperature control characteristics are analyzed.