目的 :制备抗HIVp2 4和人A型红细胞双特异性单抗 (mAb) ,并建立检测HIVp2 4的间接血凝试验。方法 :将分泌抗HIVp2 4mAb的杂交瘤株 2 E4和分泌抗人A型红细胞mAb的杂交瘤株S2 ,分别用 8 Ag和 5 BrdU驯化 ,使成为HAT敏感株。将两者常规融合 ,筛选分泌双特异性mAb的杂交 杂交瘤株。然后制备并纯化双特异性mAb ,用其建立的间接血凝法检测p2 4。结果 :共获得 6株杂交 杂交瘤细胞株 ,以其分泌的双特异性mAb建立了检测HIVp2 4的间接血凝法 ,敏感性可达 4 0 0ng/L。结论 :获得可稳定分泌双特异性mAb的杂交 杂交瘤株 ,并用纯化的双特异性mAb建立了快速检测HIVp2 4的间接血凝法 OBJECTIVE: To prepare anti-HIVp2 4 and human A-type erythrocyte bispecific monoclonal antibody (mAb) and to establish an indirect hemagglutination test for detecting HIVp24. METHODS: The hybridoma strain 2 E4 secreting anti-HIVp2 4 mAb and the hybridoma strain S2 secreting anti-human erythrocytic mAb were respectively acclimated by 8 Ag and 5 BrdU to make HAT-sensitive strains. The two were routinely fused to screen hybridoma strains secreting bispecific mAbs. Bispecific mAb was then prepared and purified, and p24 was detected using its established indirect hemagglutination assay. Results: A total of 6 hybridoma cell lines were obtained. The indirect hemagglutination assay of HIVp2 4 was established with the secreted bispecific mAb and the sensitivity was 400 ng / L. Conclusion: A hybridoma cell line that can stably secrete bispecific mAb was obtained. An indirect hemagglutination assay was developed for the rapid detection of HIVp24 using the purified bispecific mAb