论文部分内容阅读
在哮喘的发病机制中.嗜酸粒细胞(Eos)和CD_4~+T细胞的存在具有重要作用,许多细胞因子,包括TNF-α、粒细胞-巨噬细胞集落刺激因子(GM-CSF)。以及Ⅱ类细胞因子IL-4、IL-5介导了这一过程。 作者采用6～8周雌性BaLb/c小鼠相隔5天两次腹膜腔内注入0.5ml(含8μg)粘附于过氧化铝的卵白蛋白(OVA),于第二次注射后第12天给予用生理盐水稀释致5mg/ml的OVA气溶胶雾化吸入1小时,激惹小鼠。观察肺泡灌洗液(BALF)、外周血(PB)、骨髓(BM)中细胞成分的变化,以及上述细胞因子在BALF和血
In the pathogenesis of asthma, the presence of eosinophils (Eos) and CD 4+ T cells plays an important role and many cytokines, including TNF- [alpha], GM-CSF. And class II cytokines IL-4, IL-5 mediated this process. The authors used intraperitoneal injections of 0.5 ml (containing 8 μg) ovalbumin (OVA) adhering to aluminum oxide per 6 days from 6 to 8 weeks of female BaLb / c mice administered on day 12 after the second injection Mice were challenged by inhalation with OVA aerosol diluted to 5 mg / ml in saline for 1 hour. The changes of cell components in BALF, PB and BM were observed, and the changes of cytokines in BALF and blood