采用经胰导管连续灌注胶原酶技术，从每个胰腺中获得平均４６９１００±２０８３００个胰岛（７６９３个胰岛／ｇ胰腺），纯化后平均１５２０００±１０９０００个胰岛／胰腺（２４８６个胰岛／ｇ胰腺）。平均回收率３２．４９％，纯度８０％。在培养第１、第３和第７天间，胰岛细胞对高糖和茶碱刺激有明显反应，胰岛素释放量，分别为低糖的１．３９～３．６７倍，（Ｐ＜０．０５），提示分离纯化后及培养期间的胰岛功能良好。胰岛形态学观察表明，双硫腙特异性的与胰岛Ｂ细胞胰岛素颗粒内的锌螯合使其着色，台盼兰染色证实胰岛活率在９０％以上。本文将分离纯化后及培养的经双硫腙染色的阳性细胞团进行电镜检查，证实为形态结构完整的胰岛。 An average of 469100 ± 208300 islets (7693 islets / g pancreas) were obtained from each pancreas with a mean of 152000 ± 109000 islets / pancreas (2486 islets / g pancreas) after purification using a continuous catheterized collagenase technique. The average recovery rate of 32.49%, purity of 80%. In the 1st, 3rd and 7th day of culture, islet cells had obvious response to high glucose and theophylline stimulation, the insulin release amount was 1.39 ~ 3.67 times of low glucose (P <0.05) , Suggesting that the islets function well after isolation and purification and during culture. Pancreatic islet morphological observations showed that dithizone specifically chelates zinc in pancreatic B cell insulin granules, and trypan blue staining confirmed that the islet survival rate was above 90%. In this paper, after separation and purification and cultured dithizone-positive cell mass staining by electron microscopy, confirmed as morphologically intact islets.