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目的探讨中药复方“醉酒灵Ⅱ号”对急性酒精中毒小鼠血中乙醇浓度、肝中乙醇脱氢酶(al-cohol dehydrogenase,ADH)活性和脑组织中单胺神经递质的影响。方法雄性小鼠随机分为模型组和醉酒灵Ⅱ号高、中、低剂量组,模型组与醉酒灵Ⅱ号高、中、低剂量组灌酒0.01 mL/g,复制急性酒精中毒动物模型,0.5 h后给药组给予不同浓度的醉酒灵Ⅱ号,给药后用色谱法测定脑组织中单胺神经递质含量;用分光光度法测定血中乙醇浓度和肝中ADH活性。结果醉酒灵Ⅱ号组酒后1.5 h和2.5 h的血中乙醇浓度明显降低,与模型组比较有显著性差异(P<0.05)。醉酒灵Ⅱ号组酒后2.5 h肝中ADH活性虽与模型组比较无显著差异(P>0.05),但有增强趋势。醉酒灵Ⅱ号组酒后1 h脑组织中去甲肾上腺素(NE)、3,4-二羟基苯乙酸(DOPAC)含量明显增加,与模型组比较有显著性差异(P<0.05,P<0.01);HVA含量明显减少,与模型组比较有显著性差异(P<0.05)。结论醉酒灵Ⅱ号可降低急性酒精中毒小鼠血中乙醇浓度,改善急性酒精中毒时小鼠脑组织内单胺递质的变化,具有一定解酒和对抗酒精对神经系统的毒性作用。
Objective To investigate the effects of Chinese traditional medicine compound “Drunkyl II” on blood ethanol concentration, activity of alcohol dehydrogenase (ADH) in liver and monoamine neurotransmitters in brain of acute alcoholism mice. Methods Male mice were randomly divided into model group and high-, middle-, and low-dose group of Zuijiling II, and model group was treated with 0.01 mL/g of high-, middle-, and low-dose Zucchinii II to replicate acute alcoholism. 0.5 h after administration of different concentrations of drunk spirit II, after administration, the content of monoamine neurotransmitters in brain tissue was determined by spectrophotometry, and the concentration of ethanol in blood and ADH activity in liver were determined by spectrophotometry. Results The alcohol concentration in the drunken spirit II group at 1.5 h and 2.5 h after drinking was significantly decreased, which was significantly different from that in the model group (P<0.05). Although there was no significant difference in liver ADH activity between the two groups after drinking for 2.5 h (P>0.05), there was a trend of enhancement. Norepinephrine (NE) and 3,4-dihydroxyphenylacetic acid (DOPAC) were significantly increased in the brain of drunk spirit II group 1 hour after drinking, which was significantly different from the model group (P<0.05, P< 0.01); HVA content was significantly reduced, compared with the model group was significantly different (P <0.05). Conclusion Drunken liquor II can reduce the ethanol concentration in mice with acute alcoholism and improve the changes of monoamine transmitters in the mouse brain during acute alcoholism. It has certain toxic effects of alcoholism and anti-alcohol on the nervous system.