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目的探讨染矽尘大鼠肺组织FasL表达及细胞凋亡在矽肺病理变化中的意义。方法将96只Wistar大鼠随机分为对照组和染矽尘组。对染矽尘组以暴露式气管内一次注入二氧化硅粉尘悬液(1 ml,0．2 g/kg)建立大鼠矽肺模型,对照组气管内注入1 ml灭菌生理盐水。分别于处理后1、3、7、14、21和28 d,每组分别取8只大鼠处死取肺组织,用组织芯片技术结合免疫组化SP法检测大鼠肺组织不同时间点FasL的表达;末端脱氨核苷酸转移酶末端标记技术(TUNEL)法检测大鼠肺组织细胞凋亡,并采用Image-Pro Plus Version 4．5 for windowsTM图像分析计算积分光密度。结果染矽尘组大鼠肺组织FasL在染矽尘后的7、14、21和28 d时,表达明显高于对照组,差异有统计学意义(P<0．05),14 d时表达最高为157．50±23．12;染矽尘组大鼠肺组织凋亡阳性细胞在染矽尘后的1、3、7、14、21和28 d时,明显高于对照组,差异有统计学意义(P<0．05),7和14d,分别最高为32．95±20．40和31．07±7．15。结论矽尘能导致肺组织细胞凋亡;FasL在染矽尘大鼠肺组织多种细胞中表达并介导细胞的凋亡,凋亡细胞主要以炎性细胞为主。
Objective To investigate the significance of FasL expression and apoptosis in the lung tissue of silicosis rats exposed to the pathological changes of silicosis. Methods 96 Wistar rats were randomly divided into control group and silica dust group. Silicosis rats were exposed to intratracheal instillation of silica dust suspension (1 ml, 0.2 g / kg) to establish the model of silicosis in rats, and control group was injected with 1 ml sterile saline. At 1, 3, 7, 14, 21 and 28 days after treatment, 8 rats in each group were sacrificed and the lungs were sacrificed. Tissue microarray and immunohistochemical SP method were used to detect the expression of FasL The apoptosis of lung tissue was detected by TUNEL method, and the integral optical density was calculated by Image-Pro Plus Version 4.5 for windowsTM image analysis. Results The expression of FasL in the lung tissue of rats exposed to silica dust group was significantly higher than that of the control group at 7, 14, 21 and 28 d after exposure to silica dust (P <0.05), and was significantly higher at 14 d Up to 157.50 ± 23.12; the apoptotic cells in the lungs of the rats exposed to silica dust group were significantly higher than those in the control group at 1, 3, 7, 14, 21 and 28 d after exposure to silica dust Statistical significance (P <0.05), 7 and 14d, respectively, up to 32.95 ± 20.40 and 31.07 ± 7.15. Conclusion Silica dust can cause apoptosis of lung tissue cells. FasL is expressed in many kinds of cells in the lung tissues of rats exposed to silica dust and mediates cell apoptosis. The main apoptotic cells are mainly inflammatory cells.