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为了解云南省非脊髓灰质炎(脊灰)肠道病毒(NPEV)的基因型分布及分子进化特征,对2006～2010年间从急性迟缓性麻痹(AFP)病例中分离到的105株NPEVs进行VP1区部分核苷酸扩增和序列测定。所获得的云南地方株基因序列与各基因型原型株进行核苷酸与氨基酸同源性比较,并与GenBank中选取的代表株构建基因进化关系树。结果分析显示:105株NPEVs分别属于HEV-A、HEV-B、HEV-C,其中HEV-A 18株(7个血清型)所占比例为17.1%;HEV-B 77株(22个血清型)所占比例为73.3%,表明云南省AFP病例中流行的NPEV还是以HEV-B为主;HEV-C 10株(4个血清型)所占比例为9.5%;没有分离到HEV-D组肠道病毒;基因进化树中各种血清型病毒与对应原型株及代表株聚集一起,除CA2、EV90和EV76外,云南地方株与原型株位于不同分支。相同型别的毒株在5年的流行过程中变异程度亦不同,亲缘关系远近不一,表明这些病毒在云南省存在不同的传播链。
To understand the genotypic distribution and molecular evolution of non-poliomyelitis (polio) enterovirus (NPEV) in Yunnan province, 105 NPEVs isolated from AFP cases from 2006 to 2010 were used to detect VP1 Region Nucleotide Amplification and Sequencing. The nucleotide sequences and amino acid homology of Yunnan local strains were compared with those of the genotypes. The phylogenetic tree was constructed with the representative strains selected from GenBank. The results showed that 105 strains of NPEVs belonged to HEV-A, HEV-B and HEV-C, respectively. Among them, 18 (17 serotypes) HEV-A strains accounted for 17.1% ) Accounted for 73.3% of the cases, indicating that the prevalence of AFP in Yunnan Province is still dominated by HEV-B; HEV-C 10 strains (4 serotypes) accounted for 9.5%; did not separate HEV-D group Intestinal virus; all kinds of virus in the phylogenetic tree were clustered together with the corresponding prototypical strains and representative strains. Except CA2, EV90 and EV76, Yunnan local strains and prototype strains were located in different branches. The same type of strains in the five-year epidemic of variation is also different, the genetic distance is different, indicating that these viruses in Yunnan Province, there are different transmission chain.