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目的:建立愈伤平胶囊中芍药苷的含量测定方法。方法:采用高效液相色谱法测定,Dikma DiamonsilC18柱,以乙腈-0.1%磷酸(17∶83)为流动相,流速1.0mL/min,柱温:30℃,检测波长:230nm。结果芍药苷的线性范围为0.2112～2.1120μg;平均回收率为99.36%,RSD为1.96%(n=9);结论:所用方法测定样品灵敏度高,重复性好,可用于愈伤平胶囊的质量控制。
Objective: To establish a method for the determination of paeoniflorin in callus capsule. Methods: The column was Dikma Diamonsil C18 with acetonitrile-0.1% phosphoric acid (17:83) as mobile phase and the flow rate was 1.0 mL / min. The column temperature was 30 ℃ and the detection wavelength was 230 nm. Results The linear range of paeoniflorin was 0.2112 ~ 2.1120μg with the average recovery of 99.36% and the RSD of 1.96% (n = 9). Conclusion: The method has high sensitivity and good reproducibility and can be used for the quality of callus control.