论文部分内容阅读
本研究通过体外用药、体外用给药小鼠血清及在体用药三种方式，以酒精制成胚胎发育迟缓模型，以气血双补方八珍汤作对照，应用补肾阴方左归丸（汤），对体外培养的小鼠２细胞期（２ＣＥ）至囊胚期胚胎发育进行了动态观察。结果表明：用给药小鼠血清或在体应用左归汤者，体外培养的２ＣＥ至囊胚各期胚胎的发育率均显著高于酒精模型组，八珍汤组各期胚胎发育率略高于模型组，但无显著性差异；体外用药对早期胚胎发育未见明显作用。
In this study, the model of fetal growth retardation was established by alcohol in vitro, in vitro administration of mouse serum, and in vivo. The Qixue Shuangfang Bazhen Decoction was used as a control and the Bushen Yinfang Zuogui Pills (soup) was applied. The dynamic development of mouse embryos at 2 cell stage (2CE) to blastocyst stage was observed. The results showed that the rate of development of 2CEs in vitro to blastocyst embryos was significantly higher than that of the alcohol model group when administered with mouse serum or left in vivo. The embryo development rate of Bazhentang group was slightly higher than that of the alcohol model group. In the model group, there was no significant difference; in vitro administration had no obvious effect on early embryonic development.