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目的探讨白细胞介素-6(IL-6)与小儿难治性癫痫发病机制之间的潜在关系。方法采用酶联免疫吸附法检测48例小儿难治性癫痫患者癫痫发作后血清和脑脊液中IL-6的含量变化。结果癫痫发作1、6 h后,癫痫组患者血清IL-6水平分别为(8.23±0.58)、(11.63±1.35)pg/ml,与正常对照组(0.75±0.48)pg/ml相比有统计学意义(P<0.05,P<0.01);癫痫发作1、6 h后,脑脊液中IL-6水平亦逐渐升高,分别为(10.35±3.06)、(19.55±2.32)pg/ml,显著高于正常对照组[(1.27±0.65)pg/ml,P<0.01];而发作后12 h,癫痫组患者血清和脑脊液中的IL-6含量趋于正常[分别为(1.05±0.66)、(3.54±1.31)pg/ml],与正常对照组相比无显著差异(P>0.05)。结论癫痫发作可诱导血清和脑脊液中IL-6表达水平的上调,这可能促进炎性损伤的进一步加重,可能与小儿难治性癫痫的病情进展密切相关。
Objective To investigate the potential relationship between interleukin-6 (IL-6) and the pathogenesis of intractable epilepsy in children. Methods The levels of IL-6 in serum and cerebrospinal fluid (CSF) of 48 children with intractable epilepsy after epileptic seizure were detected by enzyme linked immunosorbent assay (ELISA). Results The levels of serum IL-6 in epileptic patients were (8.23 ± 0.58) and (11.63 ± 1.35) pg / ml, respectively, at 1 and 6 h after epileptic seizures, which were statistically higher than those in normal controls (0.75 ± 0.48) pg / ml (P <0.05, P <0.01). The level of IL-6 in cerebrospinal fluid also increased gradually after 1 and 6 h of epileptic seizure (10.35 ± 3.06 and 19.55 ± 2.32 pg / ml, respectively) (1.27 ± 0.65) pg / ml, P <0.01]. However, IL-6 levels in serum and cerebrospinal fluid of patients with epilepsy tended to be normal 12 hours after onset (1.05 ± 0.66, 3.54 ± 1.31 pg / ml], no significant difference compared with the normal control group (P> 0.05). Conclusion Epileptic seizures can induce the up-regulation of IL-6 in serum and cerebrospinal fluid, which may promote the further aggravation of inflammatory injury, which may be closely related to the progression of refractory epilepsy in children.