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本文报道用在北欧斯堪的纳维亚地区分离的流行性出血热病毒人工感染长爪沙鼠乳鼠获得成功。将已在欧洲棕背(鼠平)体内适应的流行性肾病(NE)病毒 H(?)lln(?)s 株,脑内接种5天龄的长爪沙鼠乳鼠,从第七天起,利用两步的免疫荧光抑制法,即可在其肺、脾、肝、胰、脑、脊髓及小肠中找到病毒相关抗原,并至少持续49天。这些抗原在脏器中的分布与 NE 病毒的自然宿主欧洲棕背(鼠平)类似。NE 病毒可在乳鼠体内连续传代,第一代即有33%乳鼠受染,到第3代以后乳鼠感染率均为100%,感染度随代数逐渐增强,其第3代及第5代接种后28天的肺悬液 ID50
This article reports the success of artificially inoculating gerbils with gerbil hemorrhagic fever virus isolated in northern Scandinavia. The Neisseria gonorrhoeae virus H (?) Lln (?) S strain that has been adapted to the European brown back (rat placenta) was intracerebrally inoculated with 5-day-old gerbil , Using two-step immunofluorescence inhibition, you can find in the lung, spleen, liver, pancreas, brain, spinal cord and small intestine virus-related antigens, and at least for 49 days. The distribution of these antigens in organs is similar to that of the natural host of European virus (brownback). NE virus can be continuously passaged in the suckling mice. In the first generation, 33% of the suckling mice were infected. By the third passage, the infection rate of the suckling mice was 100%, and the infection degree gradually increased with the generation of algebra. The third generation and the fifth On day 28 after inoculation lung suspension ID50