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河北省唐山市卫生防疫站尹卫东同志于1983年10月自一个甲型肝炎(简称甲肝)暴发流行村采取了早期病人大便和双份血清。1984年3月起,在中国预防医学中心病毒学研究所有关人员指导下,对采集标本进行了检测,检出一份含高滴度甲型肝炎病毒(HAV)的大便。将该大便悬液同时接种于2BS、CV-1、Vero细胞,在32℃下培养8周未见特异荧光。第二代传代4周后,CV-1和Vero细胞中未检出病
Comrade Yin Weidong, Tangshan City Health and Anti-epidemic Station, Hebei Province, took early patient stool and double serum from a hepatitis A outbreak epidemic village in October 1983. Since March 1984, under the guidance of the relevant personnel from the Institute of Virology of the Chinese Preventive Medicine Center, the collected samples were tested and a stool containing high-titer Hepatitis A virus (HAV) was detected. The stool suspension was seeded simultaneously in 2BS, CV-1, Vero cells, cultured at 32 ℃ for 8 weeks no specific fluorescence. Four weeks after passage 2 of the second generation, no disease was detected in CV-1 and Vero cells