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目的 观察深海鱼类角燕提取物对肝癌的抑制作用。方法 采用盐溶液抽提 ,有机溶剂分级沉淀 ,分子筛层析等步骤 ,从海洋鱼类—角燕的药用部位获得角燕提取物。选用 QGY772 1人肝癌细胞体外实验模型 ,MTT法观察角燕提取物在体外对肝癌细胞生长的抑制作用 ;选用小鼠 HAC肝癌模型 ,评价角燕提取物在体内对肝癌生长的抑制作用。结果 角燕提取物显著抑制 QGY772 1人肝癌细胞的生长 ,且呈明显的剂量依赖关系 ,但对人肺成纤维细胞 (HL F)的生长无明显影响。角燕提取物在剂量为0 .2 5～ 1.0 0 g/kg的范围内对小鼠 HAC肝癌的生长均具有显著的抑制作用 ,并呈较好的剂量依赖关系 ;在剂量为 1.0 0 g/kg时 ,对小鼠 HAC肝癌的抑瘤率为 5 5 .5 0 %。结论 角燕提取物在体内和体外均能有效地抑制肝癌的生长
Objective To observe the inhibitory effect of Anguilla anguillarum extract on liver cancer. Methods The extracts of Anguilla japonica were obtained from the medicinal parts of the marine fish Aguascophorum by salt solution extraction, organic solvent fractionation and molecular sieve chromatography. The QGY772 human liver cancer cell model was selected in vitro. MTT assay was used to observe the inhibitory effect of the extract of Anguilla japonica on the growth of hepatocellular carcinoma cells in vitro. The HAC liver cancer model was used to evaluate the inhibitory effect of Angustifolia extract on the growth of HCC in vivo. Results The extract of Anguilla japonica significantly inhibited the growth of QGY772 human hepatoma cells in a dose-dependent manner, but had no significant effect on the growth of human lung fibroblasts (HLF). The results showed that the extract of Anguilla japonica had a significant inhibitory effect on the growth of HCC hepatoma in a dose range of 0.52 ~ 1.0 g / kg in a dose-dependent manner. At a dose of 1.0 g / kg, the inhibitory rate of mouse HAC liver cancer was 55.5%. Conclusion Anguilla angulata extract can effectively inhibit the growth of liver cancer in vivo and in vitro