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目的：研究原发性肝细胞性肝癌中ｐ１６基因的状态。方法：采用多重ＰＣＲ分析法、ＳＳＣＰ分析法及以ＰＣＲ为基础的甲基化分析法分别对四川地区人群２５例原发性肝细胞性肝癌标本及其相应的癌旁组织中ｐ１６基因的缺失、突变及甲基化情况进行了检测。结果：２５例肿瘤标本中，发现３例ｐ１６基因缺失，其中２例纯合子缺失，１例半合子缺失，未发现１例突变，在未发现基因缺失的２２例肿瘤标本中发现６例有ｐ１６基因高甲基化存在。全部相应的癌旁组织中均未发现有ｐ１６基因缺失、突变或甲基化存在。结论：ｐ１６基因的异常改变在原发性肝癌的发生与发展中可能起重要作用，其改变以甲基化为主。
Objective: To study the status of p16 gene in primary hepatocellular carcinoma. METHODS: Multiplex PCR analysis, SSCP analysis, and PCR-based methylation analysis were used to isolate the p16 gene in 25 patients with primary hepatocellular carcinoma and corresponding para-carcinoma tissues in the Sichuan area. Mutation and methylation were tested. Results: Of the 25 tumor specimens, 3 were found to have deletions of p16 gene, of which 2 were homozygous and 1 was hemizygous. No mutation was found in 1 case. 6 cases were found to have p16 in 22 tumors with no gene deletion. Hypermethylation of the gene exists. No p16 gene deletion, mutation, or methylation was found in all corresponding paracancerous tissues. Conclusion: Abnormal changes in p16 gene may play an important role in the occurrence and development of primary hepatocellular carcinoma. Methylation is the main change.