论文部分内容阅读
目的 :了解小鼠高、低转移性肝癌细胞系Hca/16A3 F(F)和Hca/A2 P(P)发生微卫星不稳定性(MSI)情况 ,探讨它们与肝癌发生及转移之间的关系。方法 :随机选择3号和16号染色体上15个多态微卫星标记 ,采用聚合酶链式反应 简单重复序列多态性(SSLP)和单链构象多态性(SSCP)方法对F和P细胞系进行分析。结果 :F和P细胞系有信息的微卫星位点其等位基因与C3H相同 ,且存在多位点MSI。结论 :该肝癌细胞系3号染色体的MSI在肝癌发生中起重要作用 ,16号染色体的MSI与肝癌转移密切相关。
OBJECTIVE: To investigate the microsatellite instability (MSI) in Hca / 16A3 F (F) and Hca / A2 P (P) cell lines in high and low metastatic HCC cell lines and to investigate their relationship with the occurrence and metastasis of HCC . Methods: Fifteen polymorphic microsatellite markers were randomly selected on chromosomes 3 and 16. FSP and P cells were detected by polymerase chain reaction-simple sequence repeat polymorphism (SSLP) and single-strand conformation polymorphism (SSCP) Department for analysis. RESULTS: The informative microsatellite loci of the F and P cell lines had the same allele as C3H, and the presence of multilocus MSI. Conclusion: The MSI of chromosome 3 on the hepatocellular carcinoma cell line plays an important role in the development of hepatocellular carcinoma. MSI on chromosome 16 is closely related to the metastasis of hepatocellular carcinoma.