论文部分内容阅读
目的:观察ｒａｓＰ２１、ＧＳＴ－π在胃癌及癌前病变组织中的表达,探讨复合表达在胃粘膜癌变过程中的意义。方法:应用免疫组化(Ｓ－Ｐ)法测定４０例肠上皮化生(不全结肠型),７６例异型增生(轻度２０例,中度３４例,重度２２例)和４２例胃癌组织中ｒａｓＰ２１、ＧＳＴ－π的表达及复合表达情况,以１０例萎缩性胃炎胃粘膜做对照。结果:(１)ｒａｓＰ２１、ＧＳＴ－π在萎缩性胃炎中无阳性表达,在肠上皮化生、异型增生及胃癌中表达率及表达强度逐渐增高(Ｐ＜０．０５或０．０１);在轻、中、重度异型增生中表达率及表达强度逐渐增高(Ｐ＜０．０５或０．０１);在重度异型增生中表达率及表达强度与胃癌无显著性差异(Ｐ均＞０．０５)。(２)ｒａｓＰ２１、ＧＳＴ－π在肠上皮化生、异型增生及胃癌中复合表达率逐渐增高(Ｐ＜０．０１);在轻、中、重度异型增生中复合表达率逐渐增高(Ｐ＜０．０５);在重度异型增生与胃癌的复合表达率无显著差异(Ｐ＞０．０５)。结论:ｒａｓＰ２１、ＧＳＴ－π均与胃粘膜癌变过程有关,联合检测对胃癌的早期诊断有重要意义。
OBJECTIVE: To observe the expression of rasP21 and GST-π in gastric cancer and precancerous lesions, and to explore the significance of complex expression in the carcinogenesis of gastric mucosa. Methods: 40 cases of intestinal metaplasia (incomplete colon type), 76 cases of dysplasia (mild 20 cases, moderate 34 cases, severe 22 cases) and 42 cases of gastric carcinoma were detected by immunohistochemistry (S-P) rasP21, GST-π expression and composite expression of 10 cases of atrophic gastritis gastric mucosa as a control. Results: (1) There was no positive expression of rasP21 and GST-π in atrophic gastritis, and the expression rate and expression intensity in intestinal metaplasia, dysplasia and gastric cancer were gradually increased (P <0.05 or 0.01) The expression rate and expression intensity in mild, moderate and severe dysplasia increased gradually (P <0.05 or 0.01), while there was no significant difference between severe dysplasia and gastric cancer (P> 0.05) ). (2) The expression rates of rasP21 and GST-π in intestinal metaplasia, dysplasia and gastric cancer gradually increased (P <0.01), and the expression rate of complex in mild, moderate and severe dysplasia increased gradually (P <0 .05). There was no significant difference in the rate of compound expression between severe dysplasia and gastric cancer (P> 0.05). Conclusion: Both rasP21 and GST-π are involved in the carcinogenesis of gastric mucosa. The combined detection of these two factors is of great significance in the early diagnosis of gastric cancer.