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目的探讨糖原合成酶激酶-3β(GSK-3β)及其磷酸化产物(p-GSK-3β)在难治性癫癎(RE)患者颞叶前部的表达。方法应用逆转录-聚合酶链反应(RT-PCR)、实时荧光定量(FQ)PCR、免疫组化及免疫印迹法检测36例RE患者颞叶前部GSK-3β及其p-GSK-3β的mRNA和蛋白的表达,并与8例对照者进行比较。结果RE患者脑内神经元及胶质细胞GSK-3β mRNA及蛋白表达明显增多(均P<0.05),p-GSK-3β表达明显减少(P<0.05)。结论GSK-3β及其磷酸化产物表达异常,可能与RE的发生和发展有关。
Objective To investigate the expression of glycogen synthase kinase-3β (GSK-3β) and its phosphorylation product (p-GSK-3β) in the anterior temporal lobe of patients with refractory epilepsy (RE). Methods The anterior temporal lobe of GSK-3β and p-GSK-3β in 36 patients with RE were detected by reverse transcription polymerase chain reaction (RT-PCR), real-time fluorescence quantitative PCR (FQ), immunohistochemistry and immunoblotting mRNA and protein expression, and compared with 8 cases of control. Results The mRNA and protein expressions of GSK-3β in neurons and glial cells in RE patients were significantly increased (both P <0.05) and the expression of p-GSK-3β was significantly decreased (P <0.05). Conclusion The abnormal expression of GSK-3β and its phosphorylation products may be related to the occurrence and development of RE.