论文部分内容阅读
有关梨属植物的器官重建已经有过不少报道。赵惠祥使梨茎尖培养成功,沈孝善用组织培养法在三个西洋梨的繁殖上获得成功。我们在砂梨(Pyrus Pyrifolia Nakai)及西洋梨与砂梨的杂交种等九个种类的梨上,微型繁殖均获得成功。现将结果简报于后。一、材料与方法 5月中旬取月(砂梨系统)、康德(西洋梨与砂梨杂交种)、开花梨等品种的当年生新梢,摘除叶片冲洗数次,用酒棉在表面擦一下,然后用0.1％的昇汞液消毒10分钟,以每个腋芽为单位,剪取1.5—2.0厘米的节段作外植体,分别培养在1/2MS基本培养基上,另加ZT2mg/L、BA5mg/L、GA2mg/L,以及0.5mg/L的NAA,碳源用普通白砂糖。有些节段接后3—5天培养基褐化,此时就转换一下相同成份的培养基。
There have been many reports on the organ reconstruction of Pyrus. Zhao Huixiang Pear shoot tip culture success, Shen Xiao Shan tissue culture method in the success of three West pear breeding. We succeeded in micro-propagation on nine pear species, Pyrus Pyrifolia Nakai and hybrids of pear and pear. Now the results briefed later. First, materials and methods In mid-May to take the month (sand pear system), Kant (pear and sand pear hybrids), flowering pears and other varieties of current year shoots, remove leaves rinse several times, wipe with wine cotton on the surface , And then disinfected with 0.1% mercuric chloride solution for 10 minutes, each axillary bud unit, cut 1.5-2.0 cm segments as explants, were cultured in 1 / 2MS basic medium, plus ZT2mg / L, BA5mg / L, GA2mg / L, and 0.5mg / L NAA, carbon source with ordinary white sugar. Some segments pick 3-5 days after the medium browning, this time on the conversion of the same composition of medium.