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为探究加州新小绥螨国内种群的最佳贮藏方法,在4、7、10℃下,以叶片+叶螨和花粉2种冷藏基质及不同冷藏时间为参试因子,研究了低温贮藏对其雌成螨存活、产卵和捕食的影响,并测试了其卵冷藏后的存活及发育情况。结果显示,温度、基质和时间3个因子均显著影响冷藏处理后加州新小绥螨雌成螨的存活率、产卵量和捕食率。在4、7、10℃下,以花粉为基质冷藏超过10 d后雌成螨存活率均低于50%,不适宜长期贮藏;以叶片+叶螨为基质冷藏分别超过20、25、30 d后雌成螨存活率显著降低,超过30 d后产卵前期较对照的1.05 d显著延长,分别达到3.07、3.14、2.29 d。在2种基质中,所有冷藏处理的单雌1周日均产卵量为1.55~2.96粒,均低于对照的2.97粒,且随冷藏时间的增加呈下降趋势;冷藏后雌成螨产卵期捕食率为每12 h 11.60~15.84头,均高于对照的11.60头。冷藏温度和时间显著影响卵的孵化率和发育历期,短期冷藏后孵化的雌螨比例与对照差异不显著。表明加州新小绥螨雌成螨适宜以叶片+叶螨为冷藏基质,短期冷藏的最适温度为4~7℃,长期冷藏最适温度为10℃;卵以4℃冷藏7 d最佳,在10℃下冷藏不宜超过2周。
In order to explore the best storage method for Neospretus asiatica in China, the storage mediums of leaves + spider mites and pollen and the different refrigerated time were used as test factors at 4, 7 and 10 ℃, Female mites survival, oviposition and predation, and tested the survival and development of their eggs after cryopreservation. The results showed that the three factors of temperature, matrix and time all significantly affected the survival rate, fecundity and prey of female Newtonsites in California after cooling storage. At 4, 7 and 10 ℃, the viability of female adult mites after pollination for more than 10 days was less than 50%, which was not suitable for long-term storage. Leaf / spider mites were stored for more than 20, 25 and 30 d The survival rate of adult female mites decreased significantly, and the pre-oviposition period was significantly longer than 1.05 d after 3.0 d, reaching 3.07, 3.14 and 2.29 d respectively after more than 30 days. In each of the two substrates, the average number of eggs laid per day for all the single females treated with cold storage was 1.55-2.96, both of which were lower than 2.97 of the control, and showed a tendency of decreasing with the increase of refrigerated storage time. The rate was 11.60 ~ 15.84 per 12 h, which was higher than the control of 11.60. The storage temperature and time significantly affected the egg hatchability and developmental duration, and the percentage of female mites hatched after short-term storage was not significantly different from the control. The results showed that the optimum temperature for short-term cold storage was 4 ~ 7 ℃ and the optimum temperature for long-term storage was 10 ℃. The optimum storage temperature for eggs was 4 ℃ for 7 days, Refrigeration at 10 ° C should not be more than 2 weeks.