环境内分泌干扰物的联合作用已经是目前研究的重点.采用重组基因酵母检测法,分别测定了双酚A(BPA)、双酚AF(BPAF)、双酚AP(BPAP)、双酚F(BPF)4种双酚化合物的雌激素活性,并依据其测定结果,按照等效浓度比,设计了浓度比分别为EC10和EC50的6种二元混合物并测定了其雌激素活性.结果表明,这4种化合物的剂量-效应关系都可以用Weibull函数有效描述,BPA、BPAF、BPAP、BPF的EC50值分别为:6.81×10-6、7.44×10-7、1.43×10-5、7.52×10-6mol.L-1,其雌激素活性大小顺序为:BPAF>BPA>BPF>BPAP.依据不同的联合作用判断方法对这4种化合物的联合效应进行判断,结果表明,相同化合物的不同混合比例可能对联合作用方式产生影响,采用DA和IA预测模型可以更加直观、方便地判断联合作用的类型,而且可以反映出不同混合比例对联合作用的影响. The combined effect of environmental endocrine disruptors has been the focus of the current research.We measured the levels of BPA, BPAF, BPAP, BPF ) Were used to determine the estrogenic activity of four bisphenol compounds. Based on their results, six binary mixtures with EC10 and EC50 were designed and their estrogenic activities were determined at equivalent concentration ratios. The results showed that The dose-effect relationship of four compounds can be effectively described by the Weibull function. The EC50 values ​​of BPA, BPAF, BPAP and BPF are respectively 6.81 × 10-6, 7.44 × 10-7, 1.43 × 10-5 and 7.52 × 10 The order of estrogen activity was BPAF> BPA> BPF> BPAP. According to different methods of joint action judgment, the combined effect of these four compounds was judged. The results showed that the different mixing ratio of the same compounds May have an impact on the mode of joint action. Using DA and IA prediction models can be more intuitive and convenient to determine the type of joint action, and can reflect the impact of different mixing ratios on the joint action.