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目的:了解外源性注意和内源性注意是否可以分离?内源性注意与哪些生理位置相关?方法:用机械毁损法毁损大鼠双侧MT,观察大鼠行为和注意能力变化。同时,电击被毁损后大鼠右侧VTA,用免疫组织化学法,采取同脑左右对比,观察大鼠相关脑区的c-fos表达,了解在毁损发生后信息的传导情况。结果:大鼠在双侧MT被机械毁损之后,3天里持续的注意能力变化与麻醉时基本相同,内源性注意完全消失;强烈的痛刺激有反应,角膜、吞咽反射存在、外源性注意存在,且这种状态呈现出不可恢复的形势。未毁损MT时,电击右侧VTA,同脑左右侧表现出有显著差异的c-fos表达;毁损双侧MT后电击右侧VTA,同脑左右侧c-fos表达基本上无差异。结论:毁损MT大鼠内源性注意消失,外源性注意依然存在,外源性注意和内源性注意可以分离。从c-fos表达上可以看出,毁损MT后电击信号没有传入大鼠脑的相应部位,这可能是导致大鼠内源性注意消失的主要原因,由此推知双侧MT是大鼠内源性注意通路上的关键位置。
OBJECTIVE: To understand whether extrinsic attention and endogenous attention can be separated or not, and which physiological locations are related to endogenous attention.Methods: The bilateral MT of rats was destroyed by mechanical destructive method, and the behavior and attention ability of rats were observed. At the same time, the rats were sacrificed and the right side of the VTA, using immunohistochemical method to take the same brain around the rat c-fos expression in relevant brain regions to understand the transmission of information after the damage occurred. Results: After the bilateral MT was mechanically damaged, the change of attentional power in 3 days was basically the same as that in anesthesia, the endogenous attention disappeared completely, the strong pain stimulated response, the cornea, swallowing reflex, exogenous Attention exists, and this situation presents an unrecoverable situation. In the absence of MT, the expression of c-fos in the right VTA and the right and left brains of striatum were significantly different from those in the untreated MT. There was no difference in the expression of c-fos on the right side of the lesion and the right side of the brain. Conclusion: The endogenous caution of MT rats disappeared, exogenous attention still exists, exogenous attention and endogenous attention can be separated. From the c-fos expression can be seen, after the destruction of MT electric shock signal did not pass into the corresponding parts of the rat brain, which may lead to the disappearance of endogenous attention in rats the main reason, infer that bilateral MT is intra-rat The source of attention to the key position on the path.