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为探索肝细胞癌变导致摄取125I－胰岛素增加的可能机理，用二乙基亚硝胺（DENA）诱发大鼠原发性肝细胞癌，动态观察癌变过程中大鼠肝脏浓聚125I－胰岛素能力增强与肝细胞病理改变之间的关系。将SD大鼠80只随机分为实验组（n=40）和对照组（n=40）。两组均饲以普通饲料，实验组按70mg/kg的剂量每周1次灌胃给予DENA。实验第6、11、15和20周，由尾静脉注射125I胰岛素溶液后1小时断头处死对照组和实验组的大鼠各10只，收集血、肝、肺、脾、肾、肌和骨，称重并测量放射性，计算% ID/g。肝组织作病理组织学检查。结果显示：所有对照组大鼠肝脏组织学观察均正常。第6周实验组肝脏表面粗糙，镜下发现肝细胞增生活跃。实验组125I－胰岛素摄取率是对照组的1.86倍；第11周肝脏颜色变浅，边缘稍钝，其中1只大鼠的肝脏呈弥漫性细小颗粒，病检表现为重度增生和肝硬变，125I－胰岛素摄取率是对照组的1.50倍；第15周，除以上的病理改变外，6只实验大鼠肝脏镜下可见肝癌细胞，125I－胰岛素摄取率是对照组的1.56倍；第20周，所有实验组的肝脏长大变形、质硬、弥漫性大小不等的灰白色结节，有坏死、液化和出血，10只实验组大鼠肝脏均发现肝癌细胞，125I－胰岛素摄取率是对照组的1.46倍。实验组与对照组肝125I－胰岛素摄取
In order to explore the possible mechanism of hepatocellular carcinogenesis leading to the uptake of 125I-insulin, rat primary hepatocellular carcinoma was induced by diethylnitrosamine (DENA), and the ability to accumulate 125I-insulin in rat liver during dynamic observation was enhanced And the relationship between the pathological changes of hepatocytes. Eighty SD rats were randomly divided into experimental group (n = 40) and control group (n = 40). Both groups were fed ordinary feed, the experimental group by 70mg / kg dose once a week by intragastric administration of DENA. At the 6th, 11th, 15th and 20th week of the experiment, 10 rats in the control group and the experimental group were decapitated 1 hour after 125II insulin solution was injected into the caudal vein to collect blood, liver, lung, spleen, kidney and muscle Bone, weigh and measure radioactivity, calculate% ID / g. Liver tissue for histopathological examination. The results showed that: All control groups of rats liver histology were normal. In the sixth week, the liver surface of the experimental group was rough, and the hepatocyte hyperplasia was found in the microscope. In the experimental group, the uptake of 125I-insulin was 1.86 times that of the control group. In the 11th week, the liver color became lighter and the edge was slightly blunt. The liver of one rat showed diffuse small particles, and the pathological examination showed severe hyperplasia and cirrhosis. 125I-insulin uptake rate was 1.50 times of that in the control group. In the 15th week, except for the above pathological changes, hepatoma cells were observed in the liver of 6 experimental rats. The uptake of 125I-insulin was 1.56 times of that of the control group. , All the experimental group grew up and deformed liver, hard, diffuse size of the gray nodules, necrosis, liquefaction and bleeding, hepatopancreas were found in the liver of 10 experimental groups, 125I-insulin uptake rate of the control group 1.46 times. Experimental group and control group liver 125I-insulin uptake