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用鲫 (2n =1 0 0 )卵与异育银鲫 (3n =1 5 6 )正常精子进行人工授精 ,产生了部分的雄核发育异育银鲫 (3n =1 5 6 )。池塘中饲养的雄核发育异育银鲫性腺不仅能成熟 ,而且也能生殖。它与父本异育银鲫回交可以产生回交子一代 (B1)。雄核发育异育银鲫、回交子一代异育银鲫与父本异育银鲫非常相似。它们均具有相同的染色体数目 (3n =1 5 6 ) ,红细胞核的体积是鲫红细胞核体积的 1 .6倍。另一方面 ,用随机扩增多态DNA(RAPD)标记检测了雌鲫、雄异育银鲫、雄核发育异育银鲫、回交子一代、雄红鲤的遗传多态性。RAPD -PCR分子遗传标记结果表明与细胞学、细胞遗传学部分研究的结果相一致。因此 ,雄核发育异育银鲫存在于鲫(♀ )与 3n =1 5 6异育银鲫 (♂ )杂交的部分F1之中。
Artificial insemination of the normal sperm with crucian carp (2n = 100) eggs and allogynogenetic crucian carp (3n = 156) gave rise to parthenogenetic silver crucian carp (3n = 156). The male gonadal gonadal gland cultured in the pond not only matures, but also reproduces. It backcrosses with the male parent silver carp (B1). Allogynogenetic silver crucian carp, backcross generation allogynogenetic crucian carp is very similar to the male parent silver crucian carp. They all have the same number of chromosomes (3n = 156), and the volume of erythrocytes is 1.6 times the volume of erythrocyte nuclei. On the other hand, RAPD markers were used to detect the genetic polymorphisms of female Carassius auratus, male Carassius auratus gibelio, male Carassius auratus gibelio, Backcross progeny and male red carp. RAPD-PCR molecular genetic markers results show that the cytology and cytogenetics part of the study results are consistent. Therefore, allogynogenetic silver crucian carp was found in F1 which crossed with 3n = 156 allogynogenetic crucian carp (♂).