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本文采用放射免疫分析和甩尾测试法观察不同频率电针对大鼠脊髓Ｐ物质（ＳＰ）释放的影响。发现在电针有效组，２Ｈｚ（低频）电针时大鼠脊髓灌流液中ＳＰ—ｉｒ明显减少，而１５Ｈｚ（中频）、１００Ｈｚ（高频）和２／１５Ｈｚ（变频）刺激时，ＳＰ—ｉｒ明显增加。在电针无效组，各种频率电针时的ＳＰ—ｉｒ测定均无明显变化。脊髓蛛网膜下腔（ｉ．ｔ．）注射高选择性非肽类ＳＰ受体拮抗剂ＲＰ６７５８０可明显阻断１５Ｈｚ、１００Ｈｚ和２／１５Ｈｚ电针镇痛，而对ＺＨＺ电针镇痛无影响。注射ＲＰ６７５８０的同分异构体ＲＰ６８６５１则不能阻断上述各频率的电针镇痛作用。鉴于中、高频和变频电针促进脊髓ＳＰ的释放，而阻断ＳＰ受体可阻断上述电针镇痛，提示电针引起大鼠脊髓中释放的ＳＰ不是参与伤害感受，而是发挥了镇痛作用．关键词
In this paper, radioimmunoassay and tail-flick test were used to observe the effect of electro-acupuncture at different frequencies on substance P (SP) release from the spinal cord in rats. It was found that SP-ir significantly decreased in EA group and EA group at 2 Hz (low frequency), while SP-ir at 15 Hz, 100 Hz and 2/15 Hz, obviously increase. In the electroacupuncture ineffective group, various frequency electro-acupuncture SP-ir determination no significant change. Spinal subarachnoid (i.t.) injection of highly selective non-peptide SP receptor antagonist RP67580 can significantly block 15Hz, 100Hz and 2 / 15Hz EA analgesia, but no effect on ZHZ EA analgesia. Injection RP67580 isomer RP68651 can not block the electroacupuncture analgesia of the above frequencies. In view of the high frequency and frequency electro-acupuncture to promote the release of spinal cord SP, and block the SP receptor can block the above electro-acupuncture analgesia, suggesting that electroacupuncture induced spinal cord SP release is not involved in nociception, but played Analgesic effect. Key words