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目的 研究人膀胱移行细胞癌 (TCC)中HPV 16 / 18型感染与c erbB2 、H ras、c myc蛋白产物表达的相互关系。方法应用免疫组织化学法检测经聚合酶链反应证实的 34例HPV 16 / 18感染阳性、2 0例HPV 16 / 18感染阴性的TCC组织和 7例正常膀胱组织中c erbB2 、H ras、c myc蛋白产物的表达 ,并经统计学处理。 结果 HPV 16 / 18感染阳性组c erbB2 、H ras、c myc蛋白产物表达阳性率分别为 5 5 .9% (19/ 34)、5 8.8% (2 0 / 34)、6 1.8% (2 1/ 34) ;HPV 16 / 18感染阴性组分别为 5 5 .0 % (11/ 2 0 )、6 5 .0 % (13/2 0 )、6 5 .0 % (13/ 2 0 ) ;正常膀胱粘膜上述 3种蛋白产物表达阳性例数依次为 0、1、0例。HPV 16 / 18感染与c erbB2 、H ras、c myc蛋白产物表达无关 (P >0 .0 5 )。c erbB2 、H ras、c myc蛋白产物阳性表达率在癌组织和正常膀胱粘膜之间有显著性差异 (P <0 .0 5 ) ,且其阳性表达率与TCC的病理分级相关 (P <0 .0 5 )。癌组织内c erbB2 与c myc蛋白产物表达呈正相关 (P <0 .0 1)。 结论 在TCC的发生、发展过程中 ,HPV 16 / 18可能不是主要通过c erbB2 、H ras、c myc蛋白产物的改变来发挥作用。c erbB2 、H ras、c myc蛋白产物的改变有可能为TCC发生的晚期事件 ,提示应注意其与临床预后的关系
Objective To investigate the relationship between HPV 16/18 infection and the expression of c-erbB2, H ras and c-myc in human bladder transitional cell carcinoma (TCC). Methods Immunohistochemistry was used to detect 34 cases of HPV 16/18 infection confirmed by polymerase chain reaction, 20 cases of HPV 16/18 negative TCC tissues and 7 cases of normal bladder tissue c erbB2, H ras, c myc The protein product was expressed and statistically processed. Results The positive rates of c-erbB2, Hs-ras and c-myc protein in HPV 16/18 infection were 55.9% (19/34), 58.8% (20/34) and 61.8% / 34). HPV 16/18 negative patients were 55.0% (11/20), 65.0% (13/2 0), 65.0% (13/2), respectively. Normal Bladder mucosa expression of the above three protein products positive cases were 0,1,0 cases. HPV 16/18 infection was not related to c erbB2, H ras, c myc protein product expression (P> 0.05). The positive rates of c-erbB2, Hs-ras and c-myc protein products were significantly different between the cancerous tissues and the normal bladder mucosa (P <0.05), and the positive rates of c-erbB2, .0 5). The expression of c-erbB2 and c-myc in cancer tissues was positively correlated (P <0.01). Conclusion HPV 16/18 may not play a role mainly in the alteration of c-erbB2, Hs-ras, c-myc protein during the development of TCC. Changes in c-erbB2, Hs-ras, c-myc protein products are likely to be late events of TCC, suggesting that their relationship with clinical prognosis should be noted