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采用反相高效液相色谱法,使用BDS-C18柱和紫外检测器,以V(甲醇)∶V(乙酸钠)=1∶2缓冲溶液(pH=4.7)为流动相,在281 nm波长处,同柱测定多菌灵和福美双,此方法的标准偏差分别为0.0871和0.0697;RSD分别为0.35%和0.28%;线性相关系数分别为0.9991和0.9991;平均回收率分别为99.96%和99.87%。
Using reversed-phase high performance liquid chromatography with a mobile phase of BDS-C18 column and UV detector with V (methanol): V (sodium acetate) = 1: 2 buffer solution , The same column determination of carbendazim and thiram, the standard deviation of this method were 0.0871 and 0.0697; RSD was 0.35% and 0.28% respectively; the linear correlation coefficients were 0.9991 and 0.9991; the average recoveries were 99.96% and 99.87% .