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目的采用HPLC法测定药材中黄褐毛忍冬皂苷甲的含量。方法采用Zorbax SB-C18色谱柱(150 mm×4.6 mm,5μm);柱温35℃;流动相为乙腈-0.1%冰醋酸溶液(62∶38);检测波长210 nm。结果黄褐毛忍冬皂苷甲进样量的线性范围为1.972～7.888μg(r=0.9999),平均加样回收率为98.2%(RSD=1.3%)。结论所建方法重复性好、专属性强,可作为黄褐毛忍冬药材质量的控制标准。
OBJECTIVE To determine the content of Lonicera saponin A in medicine by HPLC. Methods The Zorbax SB-C18 column (150 mm × 4.6 mm, 5 μm) was used. The column temperature was 35 ℃. The mobile phase was acetonitrile-0.1% acetic acid solution (62:38). The detection wavelength was 210 nm. Results The linear range of the sample volume was 1.972 ~ 7.888μg (r = 0.9999) and the average recovery was 98.2% (RSD = 1.3%). Conclusion The method has good repeatability and specificity, which can be used as the quality control standard of Chinese hanyongxiong honeysuckle.