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以TSH单克隆抗体免疫7只新西兰家兔，对免疫后家兔中抗TSH独特型抗体及其对家兔甲状腺功能的影响进行了观察。免疫25周后，家兔的T4水平和甲状腺摄碘率（TRUR）较免疫前显著降低（T416．17±5．69nmol／L比30．11±15．24nmol／L，P＜0．05），2h和4hTRUR免疫前后的差值分别为11．44％±10．38％和14．21％±12．50％（P均＜0.05）。以抗TSH单克隆抗体亲和层析柱分离免疫后兔血清，发现免疫后家兔产生TSH的抗独特型抗体和抗抗独持型抗体，以TRAb放射受体分析系统和TSH结合物的固相竞争，确认上述二种抗体分别模拟TSH和抗TSH抗体，它们对家兔的甲状腺功能可产生不同的影响。
Seven New Zealand rabbits were immunized with TSH monoclonal antibody and the effects of anti-TSH idiotypic antibody and thyroid function in rabbit after immunization were observed. After 25 weeks of immunization, T4 level and thyroid uptake rate (TRUR) in rabbits were significantly lower than those before immunization (T416.17 ± 5.69nmol / L vs 30.11 ± 15.24nmol / L, P <0.05) , 2h and 4hTRUR before and after immunization were 11.44% ± 10.38% and 14.21% ± 12.50% respectively (all P <0.05). TSH monoclonal antibody affinity chromatography column after isolation of rabbit serum after immunization, rabbit immunized rabbit was found to produce anti-TSH anti-idiotypic antibodies and anti-anti-idiotypic antibodies to TRAb radioactive receptor assay system and TSH conjugate solid Compete to confirm that the above two antibodies mimic TSH and anti-TSH antibodies, respectively, which have different effects on thyroid function in rabbits.