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目的研究不同剂量天门冬对燃煤型氟中毒大鼠血清氧化应激的影响。方法选用贵州医科大学动物实验中心提供60只(90~100)g大鼠模拟氟疫区动物模型,分为5个不同组。染氟90天股动脉取血处死大鼠,分离血清,检测血清谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)和丙二醛(MDA)。结果各模型组大鼠血清GSH-Px、T-AOC、SOD低于对照组,均有统计学意义(P<0.05)。染氟组血清GSH-Px、T-AOC、SOD明显低于各组,且均有统计学意义(P<0.05)。各天门冬组比较,随剂量增高,GSH-Px高剂量与低剂量和中剂量比较,有统计学意义(P<0.05);T-AOC、SOD逐渐增高,且均有统计学意义(P<0.05)。各模型组大鼠血清MDA高于对照组,染氟组血清MDA明显高于各组,各天门冬组比较,随剂量增加,血清MDA逐渐降低,均有统计学意义(P<0.05)。结论通过不同剂量天门冬灌胃治疗,大鼠抗氧化能力得到提升,降低机体的氧化应激产物,且高剂量效果更佳。
Objective To study the effects of different doses of Asparagus on serum oxidative stress in coal-exposed fluorosis rats. Methods Sixty (90 ~ 100) g rats were used to establish the animal model of FED in Guizhou Animal Medical Center of Guizhou Medical University and divided into five different groups. Serum levels of GSH-Px, T-AOC, SOD, and serum SOD were detected by ELISA. Malondialdehyde (MDA). Results Serum GSH-Px, T-AOC and SOD in each model group were lower than those in control group (P <0.05). Serum levels of GSH-Px, T-AOC and SOD in the fluoridated group were significantly lower than those in the other groups (P <0.05). The levels of GSH-Px were significantly lower than those of low dose and middle dose (P <0.05), while the levels of T-AOC and SOD were increased with the increase of dose (P < 0.05). Serum MDA in model group was higher than that in control group. Serum MDA in fluorosis group was significantly higher than that in each group. Compared with each group, the serum MDA gradually decreased with the increase of dose (P <0.05). Conclusion Different doses of aspartate gavage treatment can enhance the antioxidant capacity of rats, reduce the body’s oxidative stress products, and high-dose effect is better.