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为了将外源基因导入花椰菜原生质体获得转基因植株，本文研究了ＰＥＧ介导的外源基因在花椰菜下胚轴原生质体中的瞬间表达。（１）２０％ＰＥＧ将质粒ｐＢＩ２２１导入原生质体后ＧＵＳ表达比１３％ＰＥＧ导入的高，但易造成原生质体损伤。（２）热激处理增强表达，但在随后的培养过程中易造成原生质体降解。（３）原生质体状况对表达有重要影响，５ｄ龄下胚轴原生质体比８ｄ龄的表达强。（４）不同质粒及启动子表达强度不同。质粒ｐＫＩＷＩ１０１比ｐＢＩ２２１表达强３倍左右。
In order to obtain transgenic plants by introducing exogenous genes into cauliflower protoplasts, transient expression of PEG-mediated exogenous genes in hypocotyl protoplasts of cauliflower was studied in this paper. (1) 20% PEG The introduction of plasmid pBI221 into the protoplast GUS expression is higher than the introduction of 13% PEG, but easy to cause protoplast damage. (2) Heat shock treatment enhanced expression, but protoplast degradation was easily induced in the subsequent culture. (3) The protoplast status had an important influence on the expression, and the hypocotyl protoplasts at 5 d were more expressed than those at 8 d. (4) Different plasmids and promoters express different intensity. Plasmid pKIWI101 is about 3-fold stronger than pBI221.