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以奶牛粪便为堆肥基质,稻壳为调理剂,采用人工翻堆好氧高温堆肥方式,研究比较了添加NMF菌群堆肥、添加灭活NMF菌群堆肥与自然堆肥腐熟过程中各个阶段酶活性变化;结合油菜种子发芽试验,探讨堆肥酶活性变化与堆肥腐熟程度的关系.结果表明:(1)人工接种堆肥比自然堆肥高温期提前7-10d,堆肥腐熟时间缩短25d;(2)添加NMF菌群堆肥、添加灭活NMF菌群堆肥、自然堆肥过程中过氧化氢酶、脲酶、酸性磷酸酶、碱性磷酸酶、纤维素酶、多酚氧化酶活性均呈“升高-降低-升高-降低”变化趋势;同一堆肥时期,接种NMF菌群堆肥酶活性高于添加灭活NMF菌群堆肥、自然堆肥酶活性;(3)堆肥腐熟后期碱性磷酸酶、多酚氧化酶酶活性较为稳定,可以作为堆肥腐熟的参考指标.
Using cow manure as compost matrix and rice husk as conditioning agent, the paper studied the changes of enzyme activities at different stages during composting of NMF, adding composted NMF and compost of natural compost The results showed that: (1) Compared with natural composting, artificial inoculation compost increased 7 ~ 10 days earlier and composting time shortened by 25 days; (2) Adding NMF bacteria In the composting process, the activities of catalase, urease, acid phosphatase, alkaline phosphatase, cellulase and polyphenol oxidase in the course of compost composting were all increased by “increasing-decreasing-increasing High-decrease ”trend. In the same compost period, the compost activity of NMF inoculated with NMF was higher than that of composting and natural compost with addition of inactivated NMF. (3) The compost late stage alkaline phosphatase, polyphenol oxidase Activity is more stable, can be used as a reference composting maturity.