Cimicifugae Rhizoma (Sheng ma) is a Ranunculaceae herb belonging to a composite family and well known in China.has been widely used in traditional Chinese medicine.The Pharmacopoeia of the People’s Republic of China contains three varieties (Cimicifuga dahurica (Turcz.),Cimicifuga foetida L.and Cimicifuga heracleifolia Kom.) which have been used clinically as Sheng-ma.However,the chemical constituents of three components of Sheng-ma have never been documented.In this study,a rapid method for the analysis of the main components of Sheng-ma was developed using ultra-high performance liquid chromatography with quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS).The present study reveals the major common and distinct chemical constituents of C.dahurica,C.foetida and C.heracleifolia and also reports principal component and statistical analyses of these results.The components were identified by comparing the retention time,accurate mass,mass spectrometric fragmentation characteristic ions and matching empirical molecular formula with that of the published compounds.A total of 32 common components and 8 markers for different Sheng-ma components were identified.These findings provide an important basis for the further study and clinical utilities of the three Sheng-ma varieties.