Quality control of the parasitoid wasp Trichogramma brassicae(Hymenoptera:Trichogrammatidae)over 45

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Trichogramma brassicae(Bezdenko)is an important biological control agent that has been used widely against many lepidopteran pests.Commonly,colonies of Tri-chogramma are mass reared on factitious hosts such as Sitotroga cerealella(Olivier)over many generations.In this study,we evaluated the quality and performance of a colony of T.brassicae that had been reared for over 45 generations(G)using two-sex life table pa-rameters and parasitism capacity.We found that female adult longevity was significantly different among sequential generations,ranging from 5.58 ± 2.5 d(at G5)to 3.75 ±1.42 d(at G45).However,no significant difference was found in male adult longevity among different generations.Although female wasps survived longer until the 15th gener-ation,they allocated more days for egg laying at G5 and G10.The highest values of gross reproductive rate(GRR),net reproductive rate(R0),intrinsic rate of increase(r),finite rate of increase(λ)and mean generation time(T)were found in G5 and G10,which also showed significantly higher c0.No significant difference in the finite parasitism rate(ω)was found among generations up to G15.These results suggest that T.brassicae wasps held under continuous laboratory rearing can be used successfully in biological control programs until the 15th generation without any loss of quality or performance.However,laboratory mass rearing colonies declined in quality after 15 generations and we suggest that they should be rejuvenated regularly by adding field-collected parasitoids periodically.
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